Journal: Frontiers in Pharmacology

Article Title: Postbiotic muramyl dipeptide alleviates colitis via activating autophagy in intestinal epithelial cells

doi: 10.3389/fphar.2022.1052644

Figure Lengend Snippet: Antibody information.

Article Snippet: ZO-1 , Bioss inc. , bs-1329R , WB(1:1000).

Techniques:

Journal: bioRxiv

Article Title: Effects of Alternative Splicing-Specific Knockdown of Tjp1 α+ by Rbm47 on Tight Junctions Assembly during Blastocyst Development

doi: 10.1101/2023.07.18.549609

Figure Lengend Snippet: (A) The position of both isoforms of Tjp1 (exon11/12; α+/α-), the α-domain (exon20/21) and two Tjp1 α-specific (exon 19/21; w/o α and alternatively spliced exon 27/28; α-) primers used for cDNA amplification of Tjp1 isoforms. Arrows mark the position and direction of primers used for cDNA synthesis. Transcription levels of Tjp1 α+/-, Tjp1 α variant (B), and Tjp1 α+/-, Tjp1 α-, Rbm47 (C). Transcription levels of Tjp1 α+/α-, Tjp1 α-, and Rbm47, shown as relative quantification (RQ). Error bars represent mean ± standard error.

Article Snippet: Primary antibodies against RBM47 (HPA006347, Sigma-Aldrich), TJP1 α+ (HP9044; Hycult Biotechnology, Uden, Netherlands) and TJP1 α-(HP9045; Hycult Biotechnology) were diluted and incubated with the embryos overnight at 4°C.

Techniques: Amplification, Variant Assay

Journal: bioRxiv

Article Title: Effects of Alternative Splicing-Specific Knockdown of Tjp1 α+ by Rbm47 on Tight Junctions Assembly during Blastocyst Development

doi: 10.1101/2023.07.18.549609

Figure Lengend Snippet: (A) Expression and localisation of Tjp1 α+ and Tjp1 α-in the Rbm47 KD blastocyst. (B) Gene expression patterns of Tjp1 variants from 8-cell to hatching blastocyst (C) Proximity ligation assay (PLA) showing the interaction between Tjp1(mainly Tjp1 α-) and Ocln in blastocysts. 8C(8-cell), 8c-cmp (compacted 8-cell), Mo (Morula), Lt-Mo (Late morula), E-Bl (early blastocyst), Ex-Bl (Expanding-Bl), H-Bl (Hatching blastocyst). Antibodies host: Rabbit (Rb), Guinea pig (GP), mouse (m).

Article Snippet: Primary antibodies against RBM47 (HPA006347, Sigma-Aldrich), TJP1 α+ (HP9044; Hycult Biotechnology, Uden, Netherlands) and TJP1 α-(HP9045; Hycult Biotechnology) were diluted and incubated with the embryos overnight at 4°C.

Techniques: Expressing, Proximity Ligation Assay

Journal: Molecules

Article Title: Ethanolic Extract from Pteris wallichiana Alleviates DSS-Induced Intestinal Inflammation and Intestinal Barrier Dysfunction by Inhibiting the TLR4/NF-κB Pathway and Regulating Tight Junction Proteins

doi: 10.3390/molecules27103093

Figure Lengend Snippet: PWE improved the expressions of occludin and ZO-1 by JNK signaling. ( A ) Target protein expressions in mice colon. ( B ) Phosphorylated c-Jun N-terminal kinases/c-Jun N-terminal kinases (p-JNK/JNK), ( C ) zonula occludens-1/β-actin (ZO-1/β-actin), ( D ) occludin/β-actin. Data are expressed as the means ± SE ( n = 8). Bars with different letters are significantly different ( p < 0.05).

Article Snippet: Antibodies of zonula occludens-1 (ZO-1), occludin, toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), TNF receptor associated factor 6 (TRAF6), c-Jun N-terminal kinases (JNK), p38 mitogen-activated protein kinase (P38), nuclear factor-kappa B p65 protein (NF-κB), and β-actin were diluted according to the manufacturer’s instructions (Servicebio Technology Co., Ltd., Wuhan, China).

Techniques:

Journal: Molecules

Article Title: Naringin Exhibited Therapeutic Effects against DSS-Induced Mice Ulcerative Colitis in Intestinal Barrier–Dependent Manner

doi: 10.3390/molecules26216604

Figure Lengend Snippet: Effect of naringin on the expression of inflammatory cytokines in ulcerative colitis mice. ( A ) TNF-α, IL-6 and IL-1β distribution in the colons of each group (immunofluorescence analysis, 200×). ( B ) Concentration of TNF-α in the blood serum. ( C ) Concentration of IL-6 in the blood serum. Data are presented as means ± SD ( n = 3). ## p < 0.01, compared with the control group; * p < 0.05 and ** p < 0.01, compared with the model group.

Article Snippet: Rabbit polyclonal antibodies for TNF-α, IL-6, IL-1β, COX-2, iNOS, Occludin, ZO-1, goat anti-rabbit IgG secondary antibodies and Texas Red conjugated goat anti-rabbit antibody were obtained from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Immunofluorescence, Concentration Assay, Control